Programmed cell death evidence in wheat (Triticum aestivum L.) roots induced by aluminum oxide (Al2O3) nanoparticles
2017
Yanık, Fatma | Aytürk, Özlem | Vardar, Filiz
Nanoparticles have been recognized as an abiotic stress factor in the last decade. Although they are extensively used in nanotechnology, the possible effects of nanoparticles on plants are still unclear. Therefore the aim of the present study was to determine the dose dependent effects of 13 nm-sized aluminum oxide nanoparticles (Al ₂O ₃ NPs) on wheat roots correlating with programmed cell death related characteristics. Exposure to different concentrations of Al ₂O ₃ NPs (5, 25 and 50 mg ml –¹) decreased the mitotic indices dose dependently and caused chromosomal abnormalities such as c-mitosis, monopolar metaphase and stickiness after 96 h. Loss of plasma membrane integrity and irregular microtubule aggregations were determined. Nuclear deformations and TUNEL positive reactions indicating programmed cell death were also observed. Al ₂O ₃ NP-induced caspase 3, 8 and 9-like activities which control programmed cell death were observed in all concentrations. According to our results Al ₂O ₃ NPs induced programmed cell death in wheat roots after 96 h.
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