Preparative separation of flavonoids in Adinandra nitida leaves by high-speed counter-current chromatography and their effects on human epidermal carcinoma cancer cells
2009
Yuan, Erdong | Liu, Benguo | Ning, Zhengxiang | Chen, Chungang
Preparative separation of camellianins A and B in Adinandra nitida leaves, which is a flavonoid-rich plant source with great practical prospects, was conducted by high-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate-ethanol-water (5:1:5, v/v). Apigenin (their aglycone) was prepared by hydrolysing a water extract and recrystallising. The purity and identity of these flavonoids was confirmed by HPLC-ESI/MS, and ¹H and ¹³C NMR. These flavones were used in cultures of the A431 cell line. Their inhibitory effect was evaluated by using the MTT assay. The results showed that three flavones could significantly inhibit the growth of human epidermal carcinoma cancer cells in a concentration-dependent manner. After 48h of treatment, IC₅₀ values of camellianin A, camellianin B and apigenin against A431 tumour cells were 9.09μM, 12.5μM, 21.0μM, respectively.
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