Comparison of the influence of different elicitors on hyoscyamine and scopolamine content in hairy root cultures of brugmansia candida
2003
ALVAREZ, S PITTA | MARCONI, P. L. | GIULIETTI, A.
Hairy roots of Brugmansia candida were exposed to different elicitors, such as pectinase, B. candida root homogenate, Hormonema ssp. homogenate, and the acetate control buffer. Pectinase increased intracellular hyoscyamine (200––300%%) and the release of both alkaloids up to 1500%% (scopolamine) and 1100%% (hyoscyamine). However, the increment observed in both alkaloids in roots with the acetate control buffer was superior than with pectinase, obtaining increases of 700%% in hyoscyamine and 200%% in scopolamine. The B. candida root homogenate enhanced the accumulation (50––600%%) and specific production of both alkaloids (ca. 150%%). Hormonema ssp. homogenates induced different responses according to the original medium in which the fungus was cultured. The effect of each elicitor is discussed.
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