Optimization of cultivation conditions for the production of gamma-cyclodextrin glucanotransferase by Bacillus macorous
2004
Wang, F. | Du, G. | Li, Y. | Chen, J.
The production of gamma-cyclodextrin glucanotransferase (gamma-CGTase) from Bacillus macorous WSH02-06 was optimized in shake flasks using conventional sequential techniques and statistical experimental design. Effects of nutrients including carbon and nitrogen sources, cation ions, initial pH, and temperature on gamma-CGTase production were investigated. Corn starch, peptone, Mn2+, and Zn2+ were found to be essential for obtaining high gamma-CGTase activity and biomass. The promoting effect of manganese and zinc on enzyme production has not been reported previously. According to the results of orthogonal array experiment, the optimal culture medium for high gamma-CGTase activity was determined. Maximal gamma-CGTase activity obtained in the optimized culture broth was about 250 U/mL which was 10-fold higher than that obtained in the basal medium. Time course of cell growth and gamma-CGTase production in a 7 l fermenter showed that enzyme production was growth associated, and that maximum gamma-CGTase activity reached 277 U/mL in 17 h.
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