Mobility of the maize transposable element En/Spm in Arabidopsis thaliana
1993
Cardon, Guillermo H. | Frey, Monika | Saedler, H. (Heinz) | Gierl, A. (Alfons)
The autonomous element En‐1 of the maize En/Spm transposable element system is capable of frequent somatic and germinal excision in the heterologous host Arabidopsis thaliana. The pattern of En‐homologous transcripts generated in transgenic Arabidopsis resembles En transcription in maize. An excision reporter construct based on NPT‐II gene (pKEn2) can be used reliably for the isolation of En‐1 germinal revertants by seed germination on kanamycin‐containing medium. Re‐insertion after germinal excision is apparently frequent. A dSpm receptor element can be efficiently trans‐activated in Arabidopsis either by En‐1 or by expressing cDNAs of tnpA and tnpD. Excision and re‐insertion of En/Spm take place with similar characteristics as in maize. This is the first description of En/Spm transposition in Arabidopsis and the parameters analysed here suggest that transposon tagging with En should be feasible in this species.
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