Analysis of the non‐recombining Y chromosome defines polymorphisms in domestic pig breeds: ancestral bases identified by comparative sequencing
2010
Cliffe, K.M. | Day, A.E. | Bagga, M. | Siggens, K. | Quilter, C.R. | Lowden, S. | Finlayson, H.A. | Palgrave, C.J. | Li, N. | Huang, L. | Blott, S.C. | Sargent, C.A.
Sequences from 20 amplicons representing nine different loci and 11369bp from the short arm of the pig Y chromosome were compared using pools of DNA from different European and Chinese breeds. A total of 33 polymorphic sites were identified, including five indels and 28 single nucleotide polymorphisms (SNPs). Three high frequency SNPs within the coding regions of SRY were further analysed across 889 males representing 25 European and 25 Asian breeds or Lines, plus a European Line of Meishan. Two haplotypes seen to be associated with ‘European' or ‘Chinese' origin in the initial SNP discovery phase were found to be the most common in their respective groups of breeds in a more detailed genotyping study. Two further SRY haplotypes are relatively rare. One was found exclusively within Tamworth, at low frequency in Retinto, and in three Chinese breeds (Huai, Sahwutou and Xiaomeishan). The other uncommon haplotype is found exclusively in Bamajiang, two further Chinese breeds (Hangjiang Black and Longling) and two European rare breeds (Mangalica and Linderödssvin), but appears based on comparison with other suids to represent an ancestral sequence.
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