Potential vector for insertion of animal virus genes into cells of higher plants
1989
Kovgan, A.A. | Zhdanov, V.M.
The DNA of cauliflower mosaic virus strains Cabb-S and CM4-18 was cloned in plasmid vectors pBR322 and pUC19, respectively. Cabb-S derivatives that had deletions of up to approximately 250 bp within gene VII and were infectious for plants were obtained. The strains were employed to construct a CMV containing inserts consisting of a approximate 475 bp fragment of the surface protein (HBsAg) gene of hepatitis B virus (HBV) at the XhoI site of gene II. A recombinant strain of CMV was constructed with a approximate 900 bp insert of HBV (ayw) DNA encoding the HBsAg fragment. The gene segment was inserted in such fashion that the HBV and protein VII reading frames matched up. The recombinant DNA was infectious for plants and produced expression of a polypeptide immunologically related to HBsAg. A comparison was made of HBsAg-specific polypeptide synthesis in cells of the yeast S. cerevisiae DC5 and leaves of infected rape (Brassica rapa).
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