Efficiency of pretreatments for optimal enzymatic saccharification of soybean fiber
2011
Karki, Bishnu | Maurer, Devin | Jung, Stephanie
The effectiveness of several pretreatments [high-power ultrasound, sulfuric acid (H₂SO₄), sodium hydroxide (NaOH), and ammonium hydroxide (NH₃OH)] to enhance glucose production from insoluble fractions recovered from enzyme-assisted aqueous extraction processing of extruded full-fat soybean flakes (FFSF) was investigated. Sonication of the insoluble fraction at 144μmₚₚ ₍ₚₑₐₖ₋ₜₒ₋ₚₑₐₖ₎ for 30 and 60s did not improve the saccharification yield. The solid fractions recovered after pretreatment with H₂SO₄ [1% (w/w), 90°C, 1.5h], NaOH [15% (w/w), 65°C, 17h], and NH₃OH [15% (w/w), 65°C, 17h] showed significant lignin degradation, i.e., 81.9%, 71.2%, and 75.4%, respectively, when compared to the control (7.4%). NH₃OH pretreatment resulted in the highest saccharification yield (63%) after 48h of enzymatic saccharification. A treatment combining the extraction and saccharification steps and applied directly to the extruded FFSF, where oil extraction yield and saccharification yield reached 98% and 43%, respectively, was identified.
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