Quantitative Analysis of Structural Isomers of Linear Pentaamines by GC–MS
2015
Furuchi, Takemitsu | Tazawa, Ayano | Numajiri, Sachihiko | Niitsu, Masaru
Structural isomers of long linear polyamines are often highly difficult to differentially quantify due to their structural similarities. In this study, we focused on pentaamines, aminopropylcanavalmine [H₂N(CH₂)₃NH(CH₂)₄NH(CH₂)₃NH(CH₂)₄NH₂, (3-4-3-4)] and N¹-aminopropyl-N⁸-aminobutylspermidine [H₂N(CH₂)₃NH(CH₂)₃NH(CH₂)₄NH(CH₂)₄NH₂, (3-3-4-4)], which have never been separated. We analyzed a mixture of the heptafluorobutyryl derivatives of these pentaamines by gas chromatography–mass spectrometry (GC–MS) and found that the polyamine content and intensity of the unique fragment ions detected in MS spectra exhibited a strong correlation. The calibration curves of appropriate combinations of the peak intensities versus pentaamine mixing ratios showed high linearity (r > 0.99) in the mixing ratio range of 0–2.33. These data indicated that the pentaamine content in the pentaamine mixed solutions could be determined using appropriate calibration curves. Therefore, this method can potentially be applied to measure the pentaamine content of biological samples.
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