Production of neo-fructooligosaccharides using free-whole-cell biotransformation by Xanthophyllomyces dendrorhous
2010
Ning, Yawei | Wang, Jinpeng | Chen, Jing | Yang, Ne | Jin, Zhengyu | Xu, Xueming
The effects of production parameters on the biotransformation of sucrose were investigated to enhance the yield of neo-FOS by Xanthophyllomyce dendrorhous cells. Cells showed optimal β-fructofuranosidase activity at neutral pH condition and the yield of neo-FOS showed no significant differences between buffer and buffer-free systems. Cell concentration negatively affected the maximum neo-FOS yield. Sucrose concentration positively increased the maximum yield of neo-FOS. Elevating the reaction temperature to 30°C, the neo-FOS productivity increased 1.85-fold compared with that at 20°C. Meanwhile, cell age of 32h enabled the biotransformation of sucrose more efficiently. In addition, free cells exhibited a higher productivity over immobilized cells. The maximum neo-FOS concentration finally reached 227.72g/l from 400g/l sucrose under the optimal conditions.
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