Linking the response of soil microbial community structure in soils to long-term wastewater irrigation and soil depth

Irrigation with treated wastewater (TWW) has become a prevailing agricultural practice due to the scarcity of fresh water resources, which may have a significant impact on the microbial communities that are critical to many biogeochemical processes in soils. However, it is unclear whether there are links between soil microbial responses to long-term irrigation with different sources of wastewater and soil depth. Here we assess the influence of treated domestic (DTWW), leather industry (LTWW) and pharmaceutical (PTWW) wastewater on microbial communities in vertical soil profiles using high-throughput sequencing based on 16S rRNA and internal transcribed spacer (ITS) gene profiling. We found that microbial α-diversity in the vertical profiles of soils was significantly influenced by TWW irrigation. Bacteria and fungi in different soil depths showed distinct responses to TWW; irrigation with TWW markedly increased abundance of bacterial OTUs and inhibited abundance of fungal OTUs. β-diversity analysis showed that the effect of TWW irrigation on microbial communities was greater than the effect of soil depth, and microbes in subsurface soil were more sensitive to different sources of irrigation water. We also found that, based on β-diversity analysis, irrigation with treated industrial wastewater, including LTWW and PTWW, had a greater impact on microbial community structures than DTWW. TWW irrigation significantly affected the composition of indigenous soil microbial communities at different depths and might introduce exogenous microbes into the soil environment. Our work explicitly demonstrates the vertical responses of bacterial and fungal communities in soils to irrigation with TWW from different sources, which can provides insights into the microbial-dominated geochemical processes from the perspective of the entire soil profile under the context of wastewater irrigation.