A role for DNA primase in coupling DNA replication to DNA damage response

Marini, F.; Pellicioli, A.; Paciotti, V.; Lucchini, G.; Plevani, P.; Stern, D.F.; Foiani, M.

A role for DNA primase in coupling DNA replication to DNA damage response

1997

The temperature-sensitive yeast DNA primase mutant pri1-M4 fails to execute an early step of DNA replication and exhibits a dominant, allele-specific sensitivity to DNA-damaging agents. pri1-M4 is defective in slowing down the rate of S phase progression and partially delaying the G1-S transition in response to DNA damage. Conversely, the G2 DNA damage response and the S-M checkpoint coupling completion of DNA replication to mitosis are unaffected. The signal transduction pathway leading to Rad53p phosphorylation, induced by DNA damage is proficient in pri1-M4, and cell cycle delay caused by Rad53p overexpression is counteracted by the pri1-M4 mutation. Altogether, our results suggest that DNA primase plays an essential role in a subset of the Rad53p-dependent checkpoint pathways controlling cell cycle progression in response to DNA damage.

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AGROVOC Keywords

biosynthesis cell cycle cell cycle damage dna dna damage dna replication dna replication enzymes enzymology flow cytometry gene expression regulation genes genetics metabolism mitosis mitosis models mutagenesis mutagens mutants mutation mutation pharmacology phosphorylation phosphorylation saccharomyces cerevisiae temperature ultraviolet radiation

Bibliographic information

Published in

EMBO journal

Volume 16 Issue 3 Pagination 639 - 650 ISSN 0261-4189

Other Subjects

Rna nucleotidyltransferases; Biological resistance; Cell cycle progression; Dna modification; Fungal; Adverse effects; S phase; Ultraviolet rays; Protein kinases; Saccharomyces cerevisiae proteins; Methyl methanesulfonate; Pri1 gene; Blotting; Western; Biological; Protein serine-threonine kinases; Fungal; Site-directed; Methyl methanesulfonate; Interphase; Cell cycle proteins; Enzyme stability; Checkpoint kinase 2; Fungal proteins; Hydroxycarbamide; Dna primase

Language

English

Note

2019-12-05

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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A role for DNA primase in coupling DNA replication to DNA damage response

1997

AGROVOC Keywords

Bibliographic information