Assessing genetic relationships among bitter gourd (Momordica charantia L.) accessions using inter-simple sequence repeat (ISSR) markers
2007
Singh, A. K. | Behera, T. K. | Chandel, D. | Sharma, P. | Singh, N. K.
Bitter gourd or balsam pear (Momordica charantia L.) belongs to the family Cucurbitaceae and is a popular vegetable in India, having considerable nutritional, economic and medicinal importance. Only one recent report is available on the genetic diversity of bitter gourd based on molecular markers (RAPDs). In the present study, experiments with inter-simple sequence repeat (ISSR) markers revealed high polymorphism among 38 genotypes, including some commercial cultivars collected from different agro-ecological zones of India. These genotypes were analysed using 15 ISSR primers (University of British Columbia, Canada) which produced a total of 125 markers, of which 94 (74.8%) were polymorphic. The number of polymorphic markers ranged from 0 (UBC 841) to 12 (UBC 890) with a mean of 6.27 markers per primer. Pair-wise genetic distances (GD) of the 38 bitter gourd accessions, based on the 125 markers, ranged from 0.093 (‘Pusa Do Mausami’- green vs. ‘DBTG 7’) to 0.516 (‘Pusa Do Mausami’- white vs. ‘DBTG 101’) and suggested a wide genetic base for these genotypes. The present study confirmed the high degree of dissimilarity between the accessions which are, therefore, genetically distinct.
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