Shoot tips of wheat as an alternative source for regenerable embryogenic callus cultures
1996
Viertel, K. | Hess, D.
Shoot tips of Triticum aestivum L. cvs. Turbo and Nandu, both summer wheat varieties, were excised from 4 and 10 day-old seedlings, and used for induction of embryogenic callus. A modified L3 medium, supplemented with 10 micromolar 2,4-dichlorophenoxyacetic acid (2,4-D) for culture initiation, and 5 micromolar 2,4-D for subculturing, was optimal; 90% of 4 day-old 'Turbo' seedlings formed embryogenic callus. Optimal plant regeneration was achieved from callus incubated on a modified MS medium without 2,4-D, but supplemented with 2.22 micromolar 6-benzylaminopurine and 0.27 micromolar naphthaleneacetic acid. Plantlets formed via embryogenesis from all embryogenic 'Turbo' calli initiated from 4 day-old explants, with a mean number of 8 regenerants per explant. Regeneration occurred via embryogenesis only. Results obtained using 'Nandu' were within the same range.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library