Chitosan nanoparticles and quercetin modulate gene expression and prevent the genotoxicity of aflatoxin B1 in rat liver
2015
Abdel-Wahhab, Mosaad A. | Aljawish, Abdulhadi | El-Nekeety, Aziza A. | Abdel-Aiezm, Sekena H. | Abdel-Kader, Heba A.M. | Rihn, Bertrand H. | Joubert, Olivier
The aims of the current study were to prepare chitosan nanoparticles (CNPs) and to evaluate its protective role alone or in combination with quercetin (Q) against AFB₁-induce cytotoxicity in rats. Male Sprague-Dawley rats were divided into 12 groups and treated orally for 4 weeks as follow: the control group, the group treated with AFB₁ (80μg/kgb.w.) in corn oil, the groups treated with low (140mg/kgb.w.) or high (280mg/kgb.w.) dose of CNPs, the group treated with Q (50mg/kgb.w.), the groups treated with Q plus the low or the high dose of CNPs and the groups treated with AFB₁ plus Q and/or CNPs at the two tested doses. The results also revealed that administration of AFB₁ resulted in a significant increase in serum cytokines, Procollagen III, Nitric Oxide, lipid peroxidation and DNA fragmentation accompanied with a significant decrease in GPx I and Cu–Zn SOD-mRNA gene expression. Q and/or CNPs at the two tested doses overcome these effects especially in the group treated with the high dose of CNPs plus Q. It could be concluded that CNPs is a promise candidate as drug delivery enhances the protective effect of Q against the cytogenetic effects of AFB₁ in high endemic areas.
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