Microsatellites-based population analysis revealed micro-diversity in two major genepools of Brassica juncea
2022
Sudan, Jebi | Singh, Ravinder | Salgotra, Romesh Kumar
Microsatellites or simple sequence repeats (SSRs) prove to be an important marker system for genetic analysis of a diverse population. The study presents the development of SSRs from A-genome of Brassica followed by genotyping with diverse genotypes from two major gene pools of Brassica juncea. A total of 61,918 SSRs were identified, of which dinucleotide repeats were most common. SSR markers were able to amplify 269 alleles among 85 genotypes. The average number of alleles per locus and PIC value was 3.24 and 0.41, respectively. Gene diversity and heterozygosity value was also able to identify a huge variability among the genotypes from two gene pools. Dendrogram based on Euclidean index was able to group genotypes into three clusters and identified micro-diversity within European gene pool. The present study will serve as a tool in the selection of contrast genotypes for Brassica juncea breeding programs.
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