2-Amino-3,4-dimethylimidazo[4,5-f]quinoline is not detectable in commercial instant and roasted coffees
1991
Gross, G.A. | Wolleb, U.
A sensitive method has been developed for the isolation of 2-amino-3,4-dimethylimidazo[4,5-f] quinoline (MeIQ) from instant coffee and lyophilized home-brewed coffee. Sample cleanup was performed in four steps using Amberlite XAD-2, Extrelut/copper phthalocyanine-sephasorb, Sephasorb HP, and propanesulfonic acid silica/octadecyl silica. The purified extracts were then separated by high-performance liquid chromatography. To validate the procedure, picogram levels of [2-14C]MeIQ were added prior to extraction to the coffee samples; 61% and 49% of the added reference standard could be recovered in chromatographic fractions from purified instant and home-brewed coffee, as measured by liquid scintillation and Ames test mutagenicity (TA98,+S-9). At 600 and 60 ppt spiking levels, clear signals were observed allowing the estimation of the detection limit for MeIQ to be about 5-10 pg/g of coffee beans. On this basis, MeIQ was not detectable in either sample of instant or roasted coffee.
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