Tyrosinase-Immobilized MCM-41 for the Detection of Phenol
2012
Mangrulkar, Priti A. | Yadav, Renu | Meshram, Jyotsna S. | Labhsetwar, Nitin K. | Rayalu, Sadhana S.
In the present investigation, we report the immobilization of the enzyme tyrosinase on mesoporous silica material, i.e. MCM-41 to serve as a tool for the detection of phenol. The enzyme immobilized onto the MCM-41 matrix has shown to retain its activity and is quite stable. The immobilization of enzyme has been discussed, and the various factors that affect the loading of enzyme onto MCM-41 were studied and optimized. The applicability of tyrosinase-immobilized MCM-41 was then demonstrated for the detection of phenol. The lowest detectable concentration of phenol by tyrosinase-immobilized MCM-41 was observed to be 1 mg l−1. The factors influencing the detection of phenol were then studied in detail.
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