Evaluation of genetic variation in high molecular weight glutenin subunits of seed storage protein using landraces of common wheat from Pakistan
2008
Niwa, K. | Suzuki, H. | Tominaga, T. | Nasim, S. | Anwar, R. | Ogawa, M. | Furuta, Y.
SDS-PAGE was used to determine the variation in high molecular weight glutenin subunits (HMW-GS) alleles present in 170 landraces of common wheat from Sindh, Balochistan and NWFP in Pakistan. Three alleles for <i>Glu-A1</i> , six alleles for <i>Glu-B1</i> and four alleles for <i>Glu-D1</i> were detected. These included three new alleles, namely <i>Glu-B1e, Glu-B1h</i> and <i>Glu-D1n</i> , which had not previously been found in landraces of Pakistani wheat. Furthermore, <i>Glu-D1m</i> and <i>Glu-D1n</i> , which are considered to be endemic alleles in Pakistan and Afghanistan, were both detected from Balochistan. Twenty genotypes were identified based on combinations of alleles at the three <i>Glu-1</i> loci. The present study showed that Pakistani landraces of common wheat have maintained a broad diversity of HMW-GS alleles, and that they may serve as genetic resources to improve wheat varieties adapted to Pakistani climatic and edaphic conditions.
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