Halomonas plays a central role in the syntrophic community of an alkaline oil reservoir with alkali-surfactant-polymer (ASP) flooding
2020
Chen, Yu | Pan, Jie | Yun, Yuan | Zhi, Bo | Li, Guoqiang | Li, Meng | Ma, Ding
Little is known about the microbial characteristics in oil reservoirs under alkali-surfactant-polymer (ASP)-flooding. In the present study, we collected two ASP-flooding samples and two nearby water-flooding samples from the Daqing oil field and performed 16S rRNA gene sequencing and metagenomic sequencing to fill this knowledge gap. The results indicated that the highly elevated pH resulted in a simple Euryarchaeotal community and a Halomonas &Nitrincola-dominated bacterial community in the production water of the alkaline oil reservoir. In addition, we hypothesized that multiple copies of genes encoding monovalent cation/proton antiporters in Halomonas and Nitrincola, and their facultative anaerobic and movable traits, were the adaptive mechanisms responsible for their competitive growth in the alkaline oil reservoir. We also revealed a unique syntrophic community in the alkaline oil reservoir and identified the central role of Halomonas within it. The present study revealed the microbial characteristics in an alkaline oil reservoir environment formed by ASP-flooding and indicated the application potential of Halomonas in AMP-flooding and microbial enhanced oil recovery (MEOR) technology to elevate the oil recovery rate from ASP-flooded oil reservoirs.
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