A galactose-specific lectin from the red marine alga Ptilota filicina
1998
Sampaio, A.H. | Rogers, D.J. | Barwell, C.J.
A lectin from the red marine alga Ptilota filicina (PFL) was isolated by affinity chromatography on cross-linked guar gum. PFL agglutinated native and papain-treated human erythrocytes with preference for type O erythrocytes. The lectin was inhibited by galactose and its derivatives. The most potent inhibitors were p-Nitrophenyl-N-acetyl-alpha-and beta-D-galactosaminide. Porcine stomach mucin, bovine submaxillary gland mucin and asialo bovine mucin were also inhibitory. The Mr of PFL, determined by gel filtration, was 56,900. SDS-PAGE gave one band with a subunit Mr of 19,320, indicating the native protein to be a trimer of apparently identical subunits. PFL was shown to be rich in acidic and hydroxyl amino acids but low in basic amino acids. The ten N-terminal amino acids were Asx-Thr-Lys-Thr-Tyr-Leu-Leu-Ala-.
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