Direct exponential amplification and sequencing (DEXAS) of genomic DNA
1997
Kilger, C. | Paabo, S.
In order to supply a sufficient amount of template molecules for DNA sequence determination, cloning into plasmids and subsequent plasmid purification, or amplification via the PCR, are generally used. Here, we present a method -- 'direct exponential amplification and sequencing' or 'DEXAS' -- that permits direct sequence determination from whole genomic DNA and thus eliminates the need for template amplification and preparation. It relies on the simultaneous amplification of a target sequence and the determination of its sequence using dideoxyterminators in a two-step cycling reaction. DEXAS can be applied to single as well as multi-copy genomic sequences, and can easily be automated.
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