Molecular cloning and characterization of cat, gpx1 and Cu/Zn-sod genes in pengze crucian carp (Carassius auratus var. Pengze) and antioxidant enzyme modulation induced by hexavalent chromium in juveniles
2013
Li, Meng | Zheng, Yao | Liang, Hongwei | Zou, Linhu | Sun, Jiejie | Zhang, Yingying | Qin, Fang | Liu, Shaozhen | Wang, Zaizhao
Hexavalent chromium (Cr6+) is a common pollutant transient metal with high toxicity in the environment. The toxicological effects partly result from oxidative damage due to the production of excessive reactive oxygen species (ROS) in the reductive process of Cr6+. To explore the influence of ROS induced directly by Cr6+ on the oxidative stress generation and antioxidant system, the full length cDNAs of antioxidant-related genes cat, gpx1 and Cu/Zn-sod were successfully acquired from pengze crucian carp first and analyzed. Furthermore, the mRNA expression of the antioxidant genes encompassing catalase (cat), copper/zinc superoxide dismutase (Cu/Zn-sod) and glutathione peroxidase (gpx1), antioxidant enzyme activities of CAT, SOD, and GPx and total protein content were further studied in the gill, intestine and liver of pengze crucian carp (Carassius auratus var. Pengze) juveniles upon acute exposure to Cr6+ at concentrations of 0.1, 1.0, 10 and 100mg/L for 4days. Differential significant changes of the antioxidant enzymes and gene expression were observed in different tissues. The findings contribute to better understanding the antioxidant mechanisms induced by Cr6+ and selecting the organic-specific sensitive biomarkers to monitor the safety of the aquatic ecosystem.
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