Simplified Procedure for Estimating Tissue Affinity for Bound Water in Durum Wheat
1997
Rascio, A. | Ruscio, M. | Di Fonzo, N.
Bound water has been studied intensively in relation to abiotic stress tolerance, but investigations of its role as a component of drought resistance in crop plants have been hampered by lack of rapid, inexpensive, and reliable methods capable of processing large populations in a short time. We investigated the possibility of estimating thermodynamic properties of strongly bound water in wheat leaves (Triticum durum Desf.) using a simplified analysis of water sorption, which made use of 39 paired isotherm curves constructed at 5 and 20°C, and thermodynamic parameters derived from them. Tissue affinity for strongly bound water (expressed as average differential enthalpy = ΔH̄) was compared with measurements of the difference in water sorbed (DWS) by the leaves at 5 and 20°C at relative humidities of 7, 11, 33, and 43%. Measurement of DWS at 33% relative humidity gave the best estimate of Δ̄, and therefore constituted a reliable, inexpensive, simplified method for the evaluation of tissue affinity for strongly bound water. Project: Biotecnologie vegetali
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