Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR
2018
Huangfu, Heping | Xu, Wenbo | Wang, Hongkui | Dong, Qing | Guo, Hongwei | Sun, Yanting | Li, Yunxia | Gao, Wenwen | Wang, Wuqing | Zhang, Jia | Shi, Jingke | Pan, Haochun | Li, Congcong | Wang, Linkang
To better understand the prevalence of Gallibacterium anatis in different poultry species, a rapid and accurate method was developed to detect G. anatis using a TaqMan fluorescent quantitative polymerase chain reaction (qPCR). Specific primers and a TaqMan probe were designed based on the reference gtxA gene sequence. The qPCR standard curve showed a good linear relationship, and the method showed good reproducibility, sensitivity, and specificity, indicating its suitability for G. anatis identification and quantitative analysis. A comparison of the detection results in 160 clinical swab samples showed that the detection rate (54.4%) of the qPCR for G. anatis was better than that of two conventional methods: gyrB gene-based qPCR for G. anatis (51.9%) and culture-based identification (34.4%). G. anatis was detected in layer chicken (77.3%), Silkie chicken (72.7%), and duck (27.1%) with relatively high detection rates, whereas dove (8.8%) and quail (3.0%) showed lower detection rates, indicating the different prevalence of G. anatis in different fowl species.
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