Determination of α- and β-acids in hops by liquid chromatography or electromigration techniques: A critical review
2022
Duarte, Lucas Mattos | Aredes, Rafaella Silva | Amorim, Tatiane Lima | de Carvalho Marques, Flávia Ferreira | de Oliveira, Marcone Augusto Leal
Hop plays an essential role in brewing beer and its study and analysis is of paramount importance. - and -acids are considered two of the most important hop components. While -acids are associated with the bitter flavor, -acids have antimicrobial effects. This work aims to critically review the published analytical methods for - and -acids determination in hops employing separation methods in liquid medium: liquid chromatography (LC) and capillary electrophoresis (CE). The types of hop samples, the optimized protocols to extract the hop acids, and the main instrumental conditions for both LC and CE techniques are highlighted and discussed. Specific and critical aspects of the - and β-acids separation by LC and CE and some challenges in this field are raised. Several key aspects discussed in this review may be of practical importance for brewers, whether in the microbrewery or industry and for researchers in the brewing field.
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