Vicamine production in multiple shoot culture derived from hairy roots of Vinca minor
1995
Tanaka, N. | Takao, M. | Matsumoto, T.
Characteristics of regenerated plants obtained from hairy roots (Ri-transformed plants) of Vinca minor L., a producer of a pharmaceutically important indole alkaloid, vincamine, were investigated. A previously established Ri-transformed clone, Vm-101, proliferates rapidly in vitro, displays a high degree of lateral branching and rapid shoot elongation and has a growth index 2.5 times that of an untransformed plant. The addition of 2.2 micromolar benzyladenine to the culture medium increased the shoot number but did not decrease the growth index. Vincamine content in the leaves of in vitro-cultured Vm-101 was twice that in the cultured untransformed plant. These results suggest that multiple shoot culture of Ri-transformed plants may be an excellent tool for in vitro vincamine production.
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