Ultrasound-assisted dispersive liquid-liquid microextraction for the determination of seven recreational drugs in human whole blood using gas chromatography–mass spectrometry
2017
Lin, Zebin | Li, Jiaolun | Zhang, Xinyu | Qiu, Meihong | Huang, Zhibin | Rao, Yulan
Recreational drugs have large impact on public health and security, and to monitor them is of urgent demand. In the present study, ultrasound-assisted dispersive liquid-liquid microextraction combined with the detection of gas chromatography-mass spectrometry was applied to the determination of seven common recreational drugs, including amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, meperidine, methadone and ketamine in 200μL of human whole blood. A series of factors which would affect the extraction efficiency were systematically investigated, including the nature and the volume of extraction and dispersing solvents, ultrasonication time, salting-out effect and pH value. The method consumed small amount of sample. The limits of detection and limits of quantification for each analyte were 10 and 40ng/mL, respectively, and the linearity was in the range of 0.04–25μg/mL (R² higher than 0.99). Good specificity, precision (1.5–8.2% for the intra-day study and 2.6–12.8% for the inter-day study), satisfactory accuracy (85.0–117.1%) and extraction recovery (77.0–92.4%) were obtained, which makes it a high performance method for the determination of recreational drugs in human whole blood samples.
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