Introgression of Chromosome Segments of Tulipa fosteriana into T. gesneriana Detected through GISH and Its Implications for Breeding Virus Resistant Tulips
2011
Marasek-Ciolakowska, A. | Ramanna, M.S. | Tuyl, J.M van
Hybridization between Tulipa gesneriana cultivars (G, 2n=2x=24) and Darwin hybrids (T. gesneriana × T. fosteriana; 2n=2x=24) was made with the aim of transferring desirable horticultural traits from Tulipa fosteriana (F) to T. gesneriana. The genome composition and the extent of intergenomic recombination in a number of diploid progenies (2n=2x=24) was evaluated by genomic in situ hybridization technique (GISH) using differently labelled total genomic DNAs of ‘Princeps’ (T. fosteriana) and ‘Ile de France’ (T. gesneriana). In all progenies analysed, it was possible to distinguish chromosomes from both parental genomes as well as the recombinant chromosomes. The number of T. fosteriana chromosomes as well as the number and type of recombinant chromosomes differed among progenies. The total number of intact T. fosteriana chromosomes in hybrids ranged from zero to three whereas the maximum number of recombinant T. fosteriana chromosomes was five. Among recombinant chromosomes, most contained a combination of a single T. gesneriana and a single T. fosteriana fragment. The maximum number of recombinant segments per chromosome was two and their position ranged from distal to highly interstitial. The Darwin hybrids turned out to be very useful intermediate parents for introgressing the T. fosteriana germplasm into the T. gesneriana assortment.
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