Chitin synthesis during in planta growth and asexual propagation of the cellulosic oomycete and obligate biotrophic grapevine pathogen Plasmopara viticola
2002
Werner, S. | Steiner, U. | Becher, R. | Kortekamp, A. | Zyprian, E. | Deising, H.B.
PCR amplification of two CHS gene fragments of the obligate biotroph Plasmopara viticola the causal agent of downy mildew of grapevine, is described. While one fragment shows homology to fungal class IV chitin syntheses, the other fragment groups with other oomycete chitin syntheses to form a novel class of chitin syntheses most closely related to class I-III. RT-PCR experiments indicate that PvCHS1 is constitutively expressed, whereas PvCHS2 is specifically transcribed in sporangiophores and sporangia. Analyses of wheat germ agglutinin labeling patterns by confocal laser scanning microscopy show that chitin is present on the surface of hyphal cell walls during in planta growth, and of sporangiophores and sporangia
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