Total Synthesis of a Functional Designer Eukaryotic Chromosome
2014
Annaluru, Narayana | Muller, Héloïse | Mitchell, Leslie A. | Ramalingam, Sivaprakash | Stracquadanio, Giovanni | Richardson, Sarah M. | Dymond, Jessica S. | Kuang, Zheng | Scheifele, Lisa Z. | Cooper, Eric M. | Cai, Yizhi | Zeller, Karen | Agmon, Neta | Han, Jeffrey S. | Hadjithomas, Michalis | Tullman, Jennifer | Caravelli, Katrina | Cirelli, Kimberly | Guo, Zheyuan | London, Viktoriya | Yeluru, Apurva | Murugan, Sindurathy | Kandavelou, Karthikeyan | Agier, Nicolas | Fischer, Gilles | Yang, Kun | Martin, J Andrew | Bilgel, Murat | Bohutski, Pavlo | Boulier, Kristin M. | Capaldo, Brian J. | Chang, Joy | Charoen, Kristie | Choi, Woo Jin | Deng, Peter | DiCarlo, James E. | Doong, Judy | Dunn, Jessilyn | Feinberg, Jason I. | Fernandez, Christopher | Floria, Charlotte E. | Gladowski, David | Hadidi, Pasha | Ishizuka, Isabel | Jabbari, Javaneh | Lau, Calvin Y. L. | Lee, Pablo A. | Li, Xuguang (Sean) | Lin, Denise | Linder, Matthias E. | Ling, Jonathan | Liu, Jaime | Liu, Jonathan | London, Mariya | Ma, Henry | Mao, Jessica | McDade, Jessica E. | McMillan, Alexandra | Moore, Aaron M. | Oh, Won Chan | Ouyang, Yu | Patel, Ruchi | Paul, Marina | Paulsen, Laura C. | Qiu, Judy | Rhee, Alex | Rubashkin, Matthew G. | Soh, Ina Y. | Sotuyo, Nathaniel E. | Srinivas, Venkatesh | Suarez, Allison | Wong, Andy | Wong, Remus | Xie, Wei Rose | Xu, Yijie | Yu, Allen T. | Koszul, Romain | Bader, Joel S. | Boeke, Jef D. | Chandrasegaran, Srinivasan
Designer Chromosome One of the ultimate aims of synthetic biology is to build designer organisms from the ground up. Rapid advances in DNA synthesis has allowed the assembly of complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge to synthetic biologists. Annaluru et al. (p. 55, published online 27 March) designed a synthetic eukaryotic chromosome based on yeast chromosome III. The designer chromosome, shorn of destabilizing transfer RNA genes and transposons, is ∼14% smaller than its wild-type template and is fully functional with every gene tagged for easy removal.
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