DArTseq Genotyping Reveals High Genetic Diversity of Polish Rye Inbred Lines
2017
Targońska-Karasek, Małgorzata | Bolibok-Brągoszewska, Hanna | Rakoczy-Trojanowska, Monika
The assessment of genetic diversity in available germplasm is crucial for improvement. In rye (Secale cereale L.) breeding programs, the knowledge of genetic variation on molecular level could be helpful in selection of appropriate components for crossing. The aim of this study was to assess genetic diversity of 150 Polish rye inbred lines with the use of diversity arrays technology sequencing (DArTseq) genotyping and the following bioinformatic tools: Bayesian clustering, principal coordinates analysis, and neighbor‐joining clustering. Altogether, 14,892 silicoDArT and 5031 single‐nucleotide polymorphism (SNP) markers were analyzed. The average polymorphic information content values calculated for both marker types were 0.22 and 0.37 for silicoDArTs and SNPs, respectively. The presented results point to a relatively high degree of genetic diversity within Polish rye breeding material, with a mean genetic similarity of 0.15 and heterozygosity from 0.01 to 0.39. These results can be applied in rye breeding, especially for selecting parental components for cross hybridization, as well as exploiting the natural genetic variation in existing resources.
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