Isolation and antimicrobial activities of fungi derived from Nymphaea lotus and Nymphaea stellata
2018
Supaphon, Preuttiporn | Keawpiboon, Chutima | Preedanon, Sita | Phongpaichit, Souwalak | Rukachaisirikul, Vatcharin
This study aimed to isolate fungi from leaves, petals, stalks and stamens of Nymphaea lotus and Nymphaea stellata and explore their bioactive potential with respect to antimicrobial activity and their cytotoxicity on human cell lines. The totals of 210 fungal isolates were obtained from 640 segments. The highest isolation rate was found in petal segments (0.44), while stamens yielded the lowest isolation rate (0.13). One hundred and ninety–five fungal extracts were evaluated for their efficiency against ten pathogenic microorganisms by colorimetric broth microdilution tests. Cell hexane extract from Eupenicillium levitum FNL036 (FNL036CH) gave the strongest antifungal activity against Cryptococcus neoformans and Talaromyces marneffei with minimum inhibitory concentration of 0.5 μg/mL and affected cell morphology of the test microorganisms. Moreover, this extract was non–cytotoxic to human embryonic kidney 293 (293T) and human keratinocytes (HaCAT) cell lines with 50% inhibitory concentration (IC₅₀) values above 100 μg/mL. This finding confirmed that fungi derived from these two Nymphaea species were a novel source of bioactive metabolites.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by National Agricultural Library