Overexpression of Arabidopsis ICR1 gene affects vegetative growth and anthesis
2017
Li, Da-Hui | Lı, Man-Li | Yu, Xiao-Bo | Wu, Di | Xı, Huan | Lin, Yi | Caı, Yong-Ping
Arabidopsis ICR1 (AtICR1) has been identified as a specific effector of Rho family small GTPases (ROP) in Arabidopsis thaliana. Previous researches have suggested that AtICR1 could function as scaffold linking ROP GTPases with vesicle trafficking and differentiation of cell polarity. To further explore whether AtICR1 is involved in potential regulation of other developmental pathways (such as anthesis), we characterized phenotypes of transgenic Arabidopsis ectopically expressing AtICR1 gene in the present research. Using GST pull-down assays, a direct protein interaction between AtICR1 and AtROP2 was detected. Thereafter, subcellular localization showed that AtICR1 are transiently expressed in both cell cortex along plasma membrane and nucleus in tobacco epidermal cells. Furthermore, Transcriptional level of AtICR1 in stable transgenic AtICR1 lines showed 20-fold higher values compared with wild-type plants via qRT-PCR analysis. Moreover, based on the AtICR1-tagged GUS activity, it was found that the overexpression of AtICR1 was in a constitutive pattern. Interestingly, the transgenic plants overexpressing AtICR1 exhibited dwarf and delay-for-anthesis phenotypes, as well deformed appearance of leaf epidermal cells. These results provided useful information on revealing novel roles of interactions between AtICR1 and ROP GTPases in regulation of plant development.
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