Production of Lipase from Geotrichum candidum Using Corn Steep Liquor in Different Bioreactors
2014
Maldonado, Rafael Resende | Aguiar-Oliveira, Elizama | Pozza, Eduardo Luiz | Costa, Fátima Aparecida Almeida | Filho, Francisco Maugeri | Rodrigues, Maria Isabel
The production of an extracellular lipase using corn steep liquor (CSL) as the nitrogen source in the cultivation of Geotrichum candidum NRRLY-552 was evaluated. The optimized conditions in shake flasks were CSL, 8.0 % w/v, soybean oil, 0.6 % w/v, pH 7.0, 30 °C, 250 rpm, and 48 h, resulting in a maximum lipase productivity of 0.438 U mL⁻¹ h⁻¹(U = the amount of enzyme required to liberate 1 μmol of fatty acid per minute). Scale-up was evaluated with airlift and stirred tank reactors; the best conditions, respectively, were 1 vvm(volume of gas per volume of medium per minute) of aeration which resulted in 0.535 U mL⁻¹ h⁻¹(32 h) and 1 vvm and 300 rpm resulting in 0.563 U mL⁻¹ h⁻¹(16 h). To facilitate downstream processes, lipase production was also evaluated using CSL previously clarified with activated charcoal resulting in 0.275 U mL⁻¹ h⁻¹(24 h) using 12 % (w/v) of clarified CSL in shake flasks. The obtained results showed that CSL leads to similar productivity compared to peptone using the same microorganism under similar conditions. In addition the cost of fermentation medium using CSL is much lower because it is a very inexpensive by-product from corn processing.
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