Performance characteristics of the temperature-modified ISO 9308-1 method for the enumeration of Escherichia coli in marine and inland bathing waters
2018
Jozić, Slaven | Vukić Lušić, Darija | Ordulj, Marin | Frlan, Erina | Cenov, Arijana | Diković, Sonja | Kauzlarić, Vesna | Fiorido Đurković, Lara | Stilinović Totić, Jasmina | Ivšinović, Danijela | Eleršek, Nives | Vučić, Anita | Peroš-Pucar, Danijela | Unić Klarin, Branka | Bujas, Lidija | Puljak, Tatjana | Mamić, Mirna | Grilec, Dolores | Jadrušić, Marija | Šolić, Mladen
This study defines performance characteristics of the temperature-modified ISO 9308-1 method for E. coli enumeration in bathing water. After a 4-hour resuscitation period at 36 ± 2 °C, the incubation temperature was changed to 44 ± 0.5 °C. Elevated incubation temperature significantly suppressed the growth of thermo-intolerant bacteria, and enhanced the selectivity of Chromogenic Coliform Agar (CCA) up to 49.5% for inland and up to 66.0% for coastal water. Consequently, most of the selectivity-related performance characteristics are improved.Relative recovery was determined by comparing an alternative method against the reference, ISO 9308-1:2014 method, following the criteria set out in ISO 17994:2014. Temperature modification did not significantly alter the results and the methods were evaluated as “not different” for both, coastal and inland waters.Chromogenic Coliform Agar was assessed as a suitable medium for reliable E. coli enumeration in bathing water when incubated for 17–19 h at 44 ± 0.5 °C after the 4–5 h resuscitation period at 36 ± 2 °C.
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