Keefektifan Perlakuan Panas Kering dan Iradiasi UV-C untuk Mematikan Cendawan Model Microcyclus ulei
2014
Cristin, Aprida | Sinaga, Meity Suradji | Adnan, Abdul Muin
<p>This study was carried out to develop treatment methods and determine the effective dose of dry heat treatment and UV-C irradiation to kill the fungus models of <em>Microcyclus ulei</em> on soybean grains and soybean meals without damaging its nutritional content. The fungus models used in this study were <em>Colletotrichum gloeosporioides, Botryodiplodia theobromae, Fusarium oxysporum</em> f. sp.<em> niveum</em>, and<em> Sclerotium rolfsii</em>. The results showed that UV-C treatment up to 12 and 24 hours exposure time at15 and 30 cm from UV-C light was not effective to kill all fungus models in PDA. Dry heat treatment at 55 °C for 30 and 60 minutes was effective to kill cultures of <em>C. gloeosporioides, B. theobromae, </em>and<em> S. rolfsii</em> in PDA, except for <em>F. oxysporum </em>f. sp. <em>niveum</em>. Further experiments on soybean showed that dry heat treatment at 55 °C for 30 minutes was effective to kill conidia of<em> C. gloeosporioides, B. theobromae, and F. oxysporum</em> f. sp. <em>niveum</em>, without damaging its protein content. However, dry heat treatment at 60 °C for 60 minutes has been proved not effective to kill sclerotia of <em>S. rolfsii.</em> If <em>M. ulei </em>had the similar resistance as<em> S. rolfsii</em>, then it would need a higher temperature and time than used in this study to kill the fungus</p><p>Key words: <em>Botryodiplodia theobromae, Colletotrichum gloeosporioides</em>, effective doses, <em>Fusarium oxysporum</em> f. sp. <em>niveum</em>, <em>Sclerotium rolfsii</em>, soybean meals</p>
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