Assessing the relationship between airborne fungi and potential dust sources using a combined approach

Dust events impose negative socio-economic, health, and environmental impacts on vulnerable areas and reflect their sources’ physiochemical and biological characteristics. This study aimed to assess the impact of two dust sources on the concentration and diversity of airborne fungi in one of the dustiest areas in the world. This study is the first attempt to investigate the relationship between dust sources fungal community and those in airborne dust. Also, the contribution of dust sources to airborne fungi was estimated. Air masses arriving at the study area were assessed using local wind rose and the HYSPLIT model. Sampling was carried out from airborne dust at the Arvand Free Zone as target areas and soil in the dried parts of the Hor al-Azim and Shadegan wetlands as source areas to explore the relationship between fungi in the dust sources and the downwind area. The samples were analyzed in the lab to extract DNA. The internal transcribed spacer (ITS) regions of the rDNA gene were amplified using the primers ITS1F and ITS4, and then PCR products were sent to the lab for sequencing. The raw DNA data were processed using the QIIME virtual box to pick operational taxonomic units and taxonomy assignments. The most common fungi at the genus level were in the order of Penicillium > Aspergillus > Alternaria > Fusarium > Paradendryphiella > Talaromyces. The similarity between air and soil fungal genera was investigated using richness and diversity indices, the phylogenetic tree, and principal component analysis. The results showed that the community structures of ambient fungi in the Hor al-Azim and Shadegan dust sources were more similar to those on dusty days than non-dusty days. The source tracker model was used to quantify the contributions of known dust sources to airborne fungi. The results showed that the main source of airborne fungi was Hor al-Azim on dusty and non-dusty days. This study’s results can help managers identify and prioritize dust sources regarding fungal species.