Vitamin B-12-Deficiency Affects Immunoglobulin Production and Cytokine Levels in Mice
2001
Funada, Ulala | Wada, Masahiro | Kawata, Tetsunori | Mori, Kazumi | Tamai, Hiroko | Isshiki, Tomoko | Onoda, Junko | Tanaka, Nobuo | Tadokoro, Tadahiro | Maekawa, Akio
To clarify the role of B-12 in the immunological function, serum C3, IgM, IgG, IgE contents, splenocytes expression of CD4, CD8, and CD4 positive intracellular IFN-gamma and IL-4 were examined in B-12-deficient mice, and the effect of the administration of CH3-B-12 was also studied. Serum C3, IgM and IgG contents were lower in B-12-deficient mice than in the control mice. On the other hand, serum IgE content was sinificantly higher in B-12-deficient mice, and the value in CH3-B-12 administered mice, administered CH3-B-12 to B-12-deficient mice for 48 h before the end of feeding period, showed a tendency to recovery. CD4+CD8– cells and CD4+CD8–/CD4–CD8+ ratio in splenocytes were significantly higher in B-12-deficient mice than in control mice. CD4+IFN-gamma+ cells was significantly lower in B-12-deficient mice than in control mice, and CD4+IL-4+ was significantly higher in B-12-deficient mice than in control mice. These results suggest that B-12-deficiency causes CD4+CD8–T cells shift from the T helper type 1 to the T helper type 2, which participate in the IgE production and elevates CD4+CD8–/CD4–CD8+ ratio. Thus, B-12 plays a role in maintaining the immune function in mice.
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