3D modelling of the pathogenic Leptospira protein LipL32: a bioinformatics approach
2017
Kumaran, Sharmilah Kumari | Bakar, Mohd Faizal Abu | Mohd-Padil, Hirzahida | Mat-Sharani, Shuhaila | Sakinah, S. | K, Poorani | Alsaeedy, Hiba | Peli, Amira | Wei, Teh Seoh | Ling, Mok Pooi | Hamat, Rukman Awang | Neela, Vasantha Kumari | Higuchi, Akon | Alarfaj, Abdullah A. | Rajan, Mariappan | Benelli, Giovanni | Arulselvan, Palanisamy | Suresh Kumar, S.
Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira species (Leptospiraceae). LipL32 is a highly abundant lipoprotein from the outer membrane proteins (OMPs) group, which highly conserved among pathogenic and intermediate Leptospira species. Several studies used LipL32 as a specific gene to identify the presence of leptospires. This research was aimed to study on the characteristics of LipL32 protein gene code, to fill the knowledge gap concerning the most appropriate gene that can be used as antigen to detect the Leptospira. Here, we investigated the features of LipL32 in fourteen Leptospira pathogenic strains based on comparative analyses of their primary, secondary structures and 3D modeling using bioinformatics approach. Furthermore, the physicochemical properties of LipL32 in different strains have been studied, shedding light on the identity of signal peptides, as well as on the secondary and tertiary structure of the LipL32 protein, supported by 3D modelling assays. The results showed that LipL32 gene was present in all the fourteen pathogenic Leptospira strains used in this study, with limited diversity in terms of sequence conservation, hydrophobic group, hydrophilic group and number of turns (random coil). Overall, these results add basic knowledge to the characteristics of LipL32 protein compared to the identification of potential candidates of antigens in future research, allowing us to consider LipL32 protein gene for further development of effective and reliable useful antigen to detect pathogenic Leptospira species.
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