Derivation of fumonisins B1 and B2 with 6-aminoquinolyl N-hydroxysuccinimidylcarbamate
1995
Velazquez, C. | Bloemendal, C. van | Sanchis, V. | Canela, R.
Fumonisins, a group of toxins produced mainly by strains of Fusarium moniliforme Sheldon, have been associated with several diseases in animals and with esophageal cancer in humans. Disadvantages of several derivatizing agents currently used for liquid chromatography include instability of the derivatives and formation of two peaks. Therefore, 6-aminoquinolyl N-hydroxy- succinimidylcarbamate, a new derivation reagent for amino acid analysis, was investigated. The derivatives formed are stable, and 2 ng of fumonisin B1 or B2 can be detected, with a linear response up to at least 800 ng injected. The method permits detection of fumonisins in ground corn using a described cleanup procedure. The HPLC detection limit for both toxins is 2 ng, which would give an apparent detection limit of 0.26 micrograms/g.
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