Hepatoprotective activity of scopoletin, a constituent ofSolanum lyratum
1998
Kang, So Young | Sung, Sang Hyun | Park, Jong Hee | Kim, Young Choong
Scopoletin (7-hydroxy-6-methoxycoumarin), a coumarin, was isolated from the aerial part ofSolanum lyratum Thunb. by the activity-guided fractionation employing carbon tetrachloride-intoxicated primary cultured rat hepatocytes as a screening system. Its hepatoprotective activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase from carbon tetrachloride-intoxicated rat hepatocytes into the culture medium. Scopoletin significantly reduced the releases of glutamic pyruvic transaminase and sorbitol dehydrogenase from the carbon tetrachloride-intoxicated primary cultured rat hepatocytes by 53% and 58%, respectively, from the toxicity in a dose-dependent manner over concentration ranges of 1 μM to 50 μM. Further studies revealed that at the concentration of 10 μM, scopoletin significantly preserved glutathione content by 50% and the activity of superoxide dismutase by 36% and also inhibited the production of malondialdehyde to the degree as seen in the control.
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