Micropropagation of 'Holy Basil' (Ocimum sanctum Linn.) from young inflorescences of mature plants
1999
Singh, N.K. | Sehgal, C.B.
In vitro micropropagation of holy basil (Ocimum sanctum L.), an Indian medicinal herb, has been accomplished on Murashige and Skoog (MS) medium utilizing young inflorescence explants. MS supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or thidiazuron (TDZ) produced only non-morphogenetic callus. Direct multiple shoots differentiated within 2-3 weeks when explants were cultured on MS containing 6-benzyl aminopurine (BAP). Of the various levels of BAP tested, MS + BAP (1.0 mgl-1) produced the maximum number of shoots. Incorporation of indole-3-acetic acid (IAA) (0.05 mgl-1) along with BAP (1.0 mgl-1) in the culture medium showed a marked increase in the number of shoots. About 92% of the in vitro regenerated shoots rooted on MS hormone-free medium within 2-3 weeks of culture and 85% of the micropropagated plantlets could be successfully established in soil, where they grew normally.
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