A novel high-sensitive indirect competitive chemiluminescence enzyme immunoassay based on monoclonal antibody for tenuazonic acid (TeA) detection
2022
Zhou, Hongyuan | Pan, Shuli | Tan, Hongxia | Yang, Yulian | Guo, Ting | Zhang, Yuhao | Ma, Liang
Tenuazonic acid (TeA), as a naturally occurring mycotoxin found in sorghum, corn, wheat, oat, tomato, apple, rapeseed and their products, has attracted increasing attention due to its wide contamination and high dietary exposure of infants and toddlers. Traditional methods usually require large instruments and high working conditions but are not suitable for low-cost large-scale screening. Thus, a rapid, sensitive and inexpensive method for TeA detection is required. Using the highly specific TeA monoclonal antibody (McAb), a novel indirect competitive chemiluminiscence enzyme immunoassay (icCLEIA) of tenuazonic acid (TeA) detection was developed. Based on the chemiluminescence system of bis(2,4,6-trichlorophenyl)oxalate-urea peroxide-3-(4-hydroxyphenyl)propanoic acid (TCPO–CH₄N₂O·H₂O₂–PHPPA) dimer, the enhancement of luminescence intensity for TeA determination was achieved. Under the optimal conditions, this method displayed a good linear range (0.06–1.62 ng mL⁻¹) with lower IC₅₀ (0.16 ng mL⁻¹) and LOD (0.01 ng mL⁻¹/7.5 μg kg⁻¹) compared with other assays. Besides, the recoveries of spiked wheat flour and oat samples tested were 76.24–88.95% and 85.34–93.28%, respectively. The results manifested that the newly developed method with the advantages of fast and high sensitivity can be used for the trace detection of TeA in foodstuffs.
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