Evaluation on anti-adipogenic activity of flavonoid glucopyranosides from Salicornia herbacea
2012
Kong, Chang-Suk | Lee, Jung Im | Kim, You Ah | Kim, Jung-Ae | Bak, Soon Sun | Hong, Joo Wan | Park, Hee Yeon | Yea, Sung Su | Seo, Youngwan
In the present study, the bioactivity-monitored isolation of flavonoid glucopyranosides from the salt-marsh plant Salicornia herbacea was performed, and their effects on adipogenic differentiation were evaluated in 3T3-L1 adipocytes. Among the solvent-partitioned fractions from S. herbacea, the 85% aq. MeOH and n-BuOH fractions effectively reduced the levels of triglyceride accumulation, glucose consumption and adipogenic transcription factor peroxisome proliferator-activated receptor-γ (PPARγ) expression. Further purification of the n-BuOH fraction led to the isolation of two flavonoid glucopyranosides, isorhamnetin 3-O-β-d-glucopyranoside (A) and quercetin 3-O-β-d-glucopyranoside (B), as the active principles. The presence of glucopyranosides A and B in adipocyte cells effectively suppressed adipogenic differentiation. Comparative analysis indicated that adipogenic differentiation was dramatically attenuated in the presence of glucopyranoside B rather than glucopyranoside A. The anti-adipogenic activity of glucopyranosides B was confirmed by down-regulation of sterol regulatory element-binding protein 1 (SREBP1), CCAAT/enhancer-binding proteins (C/EBPα), PPARγ and the adipocyte-specific proteins. Moreover, the specific mechanism mediating the effects of glucopyranosides B was confirmed by activation of AMP-activated protein kinase (AMPK). Findings from this study should underscore the nutraceutical value of S. herbacea-derived glucopyranosides as potent anti-obesity agents via alleviation of lipid accumulation.
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