Comparison of TaqMan real time PCR with immunofluorescence for diagnosis of rabies

Bansal, K.; Singh, C.K.; Ramneek,; Deka, D.; Sandhu, B. S.; Dandale, M.; Sood, N.K.

Comparison of TaqMan real time PCR with immunofluorescence for diagnosis of rabies

2012

In the present study, sensitivity comparison of TaqMan real time PCR with immunofluorescence on 20 brain samples was evaluated. Primers and probe were got synthesised for TaqMan assay on the basis of nucleoprotein gene of rabies virus. TaqMan real time PCR was able to diagnose rabies viral RNA in 13 out of 13 immunofluorescence positive cases. Sensitivity of TaqMan real time PCR was found to be 100% when compared with gold standard test Immunofluorescence. The present study concluded that TaqMan real time PCR can be used as an additional tool on samples like secretions, excretions and skin biopsy for confirmatory diagnosis of rabies.

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AGROVOC Keywords

animal pathology biopsy brain fluorescent antibody technique genes immunofluorescence nucleoproteins quantitative polymerase chain reaction rabies rna

Bibliographic information

Published in

Indian journal of veterinary pathology

Volume 36 Issue 2 Pagination 240 - 242 ISSN 0973-970X

Publisher

Indian Association of Veterinary Pathologists

Other Subjects

Taqman real time pcr; Rabies lyssavirus

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-28

Format: MODS

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Comparison of TaqMan real time PCR with immunofluorescence for diagnosis of rabies

2012

AGROVOC Keywords

Bibliographic information