Intracellular and extracellular sources, transformation process and resource recovery value of proteins extracted from wastewater treatment sludge via alkaline thermal hydrolysis and enzymatic hydrolysis

Excess sludge contains a large amount of protein and can be recycled to prepare industrial foaming agents, foliar fertilizers and other high value-added products. The optimization and effects of sludge protein extraction using the common processes of alkaline thermal hydrolysis (ATH) and enzymatic hydrolysis (EH) have been widely studied. This study focused on the protein extraction mechanisms of ATH and EH by comparing the ratio of intracellular to extracellular proteins extracted and the transformation of protein during the hydrolysis process. The extracellular protein content was 82.6 ± 5.07 mg/g VSS, and the content of intracellular protein extracted using ATH and EH was 376.9 mg/g VSS and 127.9 mg/g VSS, respectively. The ratio of intracellular to extracellular proteins extracted by ATH and EH was 4.5 and 1.5, respectively, indicating that ATH had a much better wall-breaking effect that allowed it to extract abundant intracellular proteins. The protein content obtained from ATH continuously increased over time, and approximately 38 % of proteins were further hydrolyzed to polypeptides. In contrast, the relatively low protein content extracted by EH possibly limited subsequent polypeptide hydrolysis, but subsequent hydrolysis to amino acids was not noticeably affected and was linearly correlated with the amount of protein extracted. An analysis of the recycling convenience and value of extracted proteins showed that the sludge dewatering performance increased by 86.7 % and 45.5 % after ATH and EH treatment, respectively, which was conducive to the subsequent separation of the protein solution. The protein extracted by ATH, with a large amount of peptides, would be beneficial to prepare industrial foaming agents, while the protein extracted by EH was rich in free amino acids and could be used to prepare foliar fertilizer.