Enzymatic synthesis of eugenol benzoate by immobilized Staphylococcus aureus lipase: Optimization using response surface methodology and determination of antioxidant activity
2010
Horchani, Habib | Ben Salem, Nadia | Zarai, Zied | Sayari, Adel | Gargouri, Youssef | Chaâbouni, Moncef
The ability of a non-commercial immobilized Staphylococcus aureus lipase to catalyze the esterification of eugenol with benzoic acid was checked and the antioxidant power of the ester formed was evaluated. Response surface methodology based on four variables (the reaction temperature, the amount of lipase, the benzoic acid/eugenol molar ratio and the volume of solvent) was used to optimize the experimental conditions of eugenol benzoate synthesis. The maximum conversion yield (75%) was obtained using 240IU of immobilized lipase, a benzoic acid/eugenol molar ratio of 1.22 dissolved in 4.6ml chloroform at 41°C. The antioxidant activities of eugenol and its ester were evaluated. Compared to BHT, used as a model synthetic antioxidant, the eugenol benzoate showed a higher antioxidative activity. The IC₅₀ value for 1,1-diphenyl-2-picrylhydrazyl was found to be 18.2μg/ml versus 20.2μg/ml for eugenol and eugenol benzoate.
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