Sucrose treatment suppresses programmed cell death in broccoli florets by improving mitochondrial physiological properties
2019
Han, Airu | Cao, Shifeng | Li, Yanxia | Wang, Hongfei | Wei, Yingying | Shao, Xingfeng | Xu, Feng
The effect of sucrose treatment on mitochondrial membrane potential (ΔΨm), contents of reactive oxygen species (ROS), cytochrome c oxidase (COX) activity, alternative oxidase (AOX) activity, mitochondrial Ca²⁺ content and cytochrome c release from mitochondria in broccoli florets during storage at 20 °C was investigated. The results showed that sucrose treatment effectively retarded the onset of programmed cell death (PCD) in broccoli florets by a mitochondria-dependent pathway. Sucrose treatment inhibited the increase of ROS and a decrease in ΔΨm, which was associated with the open state of permeability transition pore (PTP) and the ratio of cytochrome c/a. The decrease of COX activity and Ca²⁺ content in broccoli florets was suppressed under sucrose treatment. Sucrose treatment maintained higher AOX activity, and retarded the cytochrome c release from mitochondria. These results indicated that sucrose treatment delayed the appearance of PCD in broccoli florets through improving mitochondrial physiological properties.
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