The develoment of codominant PCR/RFLP based markers for the flax rust-resistance alleles at the L locus
1999
Hausner, G. | Rashid, K.Y. | Kenaschuk, E.O. | Procunier, J.D.
The flax L locus exists as a single gene with at least 13 alleles with different rust-resistance specificities. With regards to resistance to North American races of flax rust the L2, L6, and L11 alleles are of major importance. Molecular markers have been developed by screening primer sets, whose sequences were based on the nucleotide sequence of L6, for their ability to amplify segments of the L gene. One primer combination was found to amplify only the L6 or L11 alleles and another primer set was found to amplify the 3' end of all important L alleles. The latter primer set yielded a 1.3 kb fragment which upon digestion with the endonuclease MboI generated RFLP patterns unique to L2, L6, L9, and L11. The application of PCR markers to a set of 22 cultivars, comprised of deregistered, recent, and yet to be released cultivars verifies genetic studies done by previous workers and demonstrates the usefulness of the markers for following segregation of L alleles in crosses amongst wide or narrow selections of cultivars. Overall, the results confirmed that L6 is present in many Canadian flax cultivars. However, in several recently-released flax cultivars that have rust resistance conditioned by genes at other loci, the L9 allele was detected. These molecular markers will be useful in marker-assisted selection and the introduction of new genes for rust resistance in the flax breeding programs.
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